The Berkeley Center for Structural Biology (BCSB) is a national user facility operating under aegis of LBNL’s Molecular Biophysics and Integrated Bioimaging Division at Lawrence Berkeley National Laboratory. An umbrella organization, the BCSB operates five protein crystallography beamlines at the Advanced Light Source (ALS). In June of 1998, BCSB staff welcomed the first research teams to Beamline 5.0.2; Beamline 5.0.1 came online in 1999, followed by Beamline 5.0.3 in 2000. Beamlines 8.2.1 and 8.2.2 began operation in 2003. Since inception the BCSB has represented an unusual partnership among industrial, academic, national laboratory, and non profit researchers. These five beamlines have been uncommonly productive when considered across the metrics of publication record, number of solved structures, and scientific merit and significance of work.