Logistics Letter

We are here to help with arrangements for your remote data collection beamtime scheduled on the BCSB Beamlines at the Advanced Light Source (ALS) on the above date. Please send responses from the following before your scheduled beamtime:

I. Logistics

An ALS Active Blanket User Agreement (s) must be fully executed, current and in place with LBNL’s Office of Sponsored Projects and Industry Partners (OSPIP) prior to your beamtime schedule. Please check to make sure the necessary agreements are registered with LBNL by going to the OSPIP website.

Remote data collection users are now required to register with the ALS. If you are not currently registered, please go to the following website .

Note: If a user is currently registered and goes onto the beamline floor once in the fiscal year (Oct 1-Sep 30), they will already be counted as a user, and do not need to complete the above remote user registration.

Shift Claims must be submitted prior to scheduled beamtime:
1. Number of shifts that will be Proprietary.
2. Number of shifts that will be Non-Proprietary.
Please provide the following information for each remote user:
1. Full Name
2. Data collection time
3. Email address
4. Cell phone or telephone during data collection

II. Dewars

How many dewars do you plan to ship?
What date do you plan to ship your dewar(s)?
What is the incoming Fed Ex tracking Number?
What is the return Fed Ex tracking number?

To ensure proper return of your dewar(s), please include two copies of a return fed ex shipping label for each dewar and specify the date of return. Place the shipping label in the dewar cap inside the shipping container. Do not place paperwork at the bottom of the dewar. Please email a pdf of the fed ex shipping label to BCSBbeamtime@lbl.gov. Your dewar will be returned to you the day of completion of your beamtime unless you notify us by 10AM that day. Dewars will not be shipped out on Fridays.

Please note: For international shipments, please provide us with your fed ex account number, address, and phone number. We will generate a fed ex label and ship your dewar(s) back. Please be sure your shipment arrives on a weekday (Monday-Friday) as LBNL Receiving is closed on Saturdays and Sundays. Please ship your dewar to arrive at LBNL at least one day prior to your beamline schedule to allow adequate time for delivery to the ALS beamlines from LBNL Central Receiving without interruption

Please ship to:

Stacey Ortega BL (Insert beamline number here)

Lawrence Berkeley National Laboratory

1 Cyclotron Rd.; MS 6R2100

Berkeley, CA 94720

(510) 495-2450

When received, your dewar will be filled with liquid nitrogen (LN). If your crystals require any special care, be sure to let us know before shipping to LBNL.
Please let us know the type of pucks you will be sending (ALS, Unipuck, Rigaku), and your priority list for pucks.

Note: For remote collection on weekends, please consolidate samples into as few pucks as possible. Puck swaps will not be possible after 1:00 pm on weekends.
Please let us know if you will be doing energy scans.
If you are new to remote data collection, you may be able to borrow pucks for your upcoming beamtime. Please contact bcsbbeamtime@lbl.gov to inquire about loaner availability.

IMPORTANT NOTE Regarding Dewars:

Please make sure proper shipping procedures are followed and that all hazards are properly marked before shipping your dewars.
Dewars declared as dry, should be free of liquid nitrogen.
Do Not send uranium derivatives, without contacting BCSB administration @ bcsbbeamtime@lbl.gov
Do Not send viable material, such as, viruses, prion proteins or biotoxins, without contacting bcsb administration @ bcsbbeamtime@lbl.gov
Please remove all old labels from your dewar before shipping.

III. Hazards (please answer all hazards questions)
Note: hazards list has been revised to comply with NIH guidelines

If the biological material contained in these crystals was produced and purified using recombinant technology, did you use other expression system than the standard ones expressed in the following hosts (E.Coli, SF9/TNi insect cells, yeast, mammalian cells Hek/Cho> (if recombinant technology was not used answer no)
Do your crystals contain any recombinant or synthetic nucleic acids (>80 nt) that is one of the following 1) associated with replication (e.g. ORF) 2) designed to integrate into DNA 3) produce a toxin 4) capable of human gene transfer 5) contained in virus or virus-like particles?
Do your crystals contain a Prion, Prion-like or TAT-fusion Protein?
Will you ship or bring radioactive substances (e.g. uranium) or any hazardous molecules (e.g. highly concentrated heavy atoms in solution, reactive chemicals) to the ALS?
Do your crystals contain any heavy or hazardous atoms (e.g. selenium, mercury, etc.)? If so, which ones?
Will you ship or bring viable (i.e. able to grow or infect) biological material or agents (e.g. cell, bacterium, virus, prion, etc.) or a toxin to ALS?

IV. User Reports

To help better serve BCSB Users, we ask that you take a few moments to complete the ALS User Satisfaction Report

All publications based on work done in whole, or in part, at the ALS/BCSB should include the following acknowledgment:

The Berkeley Center for Structural Biology is supported in part by the National Institutes of Health, National Institute of General Medical Sciences, and the Howard Hughes Medical Institute. The Advanced Light Source is supported by the Director, Office of Science, Office of Basic Energy Sciences, of the U.S. Department of Energy under Contract No. DE-AC02-05CH11231. The Pilatus detector was funded under NIH grant S10OD021832. The ALS-ENABLE beamlines are supported in part by the National Institutes of Health, National Institute of General Medical Sciences, grant P30 GM124169

Authors of publications are required to report all ALS-related work, i.e., journal article, conference presentation, book or book chapter, theses, contributed news articles, internal staff reports, general ALS publications, brochures, etc. at Report Publications, Awards, Talks.

Please let us know if you have any questions or concerns. We are looking forward to your upcoming beamtime collaboration!

Thank you,

Stacey Ortega

Senior Administrator (510) 495-2450

Website: http://bcsb.lbl.gov