X-Ray Footprinting: Current Science and Data Processing How-To

X-Ray Footprinting For Structural Biology

Organizers: Corie Ralston and Sayan Gupta

ALS Users’ Meeting
Date: Oct 5, 2016
Venue: Lawrence Berkeley National Laboratory, 6-1105

Workshop Description

X-ray Footprinting (radiolytic hydroxyl radical labeling and mass spectrometry) is a technique that provides structural and dynamical information on proteins in the solution state and is highly complementary to many other structural and biochemical techniques. Because it yields residue-specific information by in-situ generation of hydroxyl radicals, it is used to determine conformation changes, ligand binding and protein-protein interactions under various conditions at a high degree of both temporal and spatial resolution and therefore is useful to both fundamental research and characterization of biologics/protein therapeutics. The technique was pioneered at the NSLS a decade ago and is now available at the ALS. This workshop will provide an overview of the X-Ray footprinting technique, how it is performed, the best way to prepare samples, and what information the technique provides. The morning session will highlight recent case studies, and the afternoon session is a tutorial on data-processing. The workshop will be useful for biological researchers in both academia and the pharmaceutical industry.

Agenda