{"id":614,"date":"2026-10-27T13:08:25","date_gmt":"2026-10-27T20:08:25","guid":{"rendered":"https:\/\/bcsb.als.lbl.gov\/?page_id=614"},"modified":"2026-10-27T16:44:03","modified_gmt":"2026-10-27T23:44:03","slug":"serial-crystallography-collection","status":"publish","type":"page","link":"https:\/\/bcsb.als.lbl.gov\/?page_id=614","title":{"rendered":"Serial Crystallography Collection"},"content":{"rendered":"\n

BCSB Serial crystallography at the ALS<\/h3>\n\n\n\n

The BCSB and ALS are developing serial crystallography for ligand screening and time-resolved experiments as a capability at Gemini beamline 2.0.1, in collaboration with the Kern<\/a>, Brewster<\/a>, and Weber<\/a> groups. Users can contact beamline staff to run experiments on a trial basis as the technique becomes standardized.<\/p>\n\n\n\n

Approach<\/h3>\n\n\n\n

We use a tape drive sample delivery system: a suspension of crystals are ejected as droplets onto a Kapton tape, which moves the droplets to a second injector which injects tiny droplets of substrate\/ligand.  Rapid diffusion enables ligand binding as the droplets move to the X-ray beam (here marked as XFEL, but usable at synchrotrons as well).<\/p>\n\n\n\n

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Sample requirements<\/h3>\n\n\n\n

A single dataset typically requires 3000 to 10000 indexed images, which depending on the hit rate (number of indexed images as a percent of the total images collected), can take 3 to 15 minutes to collect.  Given typical flow rates of 12 microliters per minute of sample and ligand, sample consumption can be from 30 to 200 uL. For example:<\/p>\n\n\n\n

Dataset size<\/td>Hitrate<\/td>Collection speed (images per second)<\/td>Dataset time (minutes)<\/td>Sample and ligand consumption @ 12 uL\/min (ul)<\/td>Sample consumption for lysozyme, @ 14.4 kDa, ~1.75 mM (mg)<\/td><\/tr>
3000<\/td>10%<\/td>100<\/td>10<\/td>120<\/td>3<\/td><\/tr>
3000<\/td>20%<\/td>100<\/td>2.5<\/td>30<\/td>0.75<\/td><\/tr>
10000<\/td>10%<\/td>100<\/td>17<\/td>200<\/td>5<\/td><\/tr>
10000<\/td>20%<\/td>100<\/td>8<\/td>100<\/td>2.5<\/td><\/tr><\/tbody><\/table><\/figure>\n\n\n\n

For more detail and to consult about possible experiments, please contact beamline staff (Daniil Prigozhin, DaniilPrigozhin@lbl.gov<\/a>).<\/p>\n\n\n\n

References<\/h3>\n\n\n\n

Butryn A. et al.<\/em> (2021). “An on-demand, drop-on-drop method for studying enzyme catalysis by serial crystallography.” Nat Commun<\/em> 12<\/strong>, 4461. https:\/\/dx.doi.org\/10.1038\/s41467-021-24757-7<\/a><\/p>\n\n\n\n

<\/p>\n","protected":false},"excerpt":{"rendered":"

BCSB Serial crystallography at the ALS The BCSB and ALS are developing serial crystallography for ligand screening and time-resolved experiments as a capability at Gemini beamline 2.0.1, in collaboration with the Kern, Brewster, and Weber groups. Users can contact beamline staff to run experiments on a trial basis as the technique becomes standardized. Approach We… Continue reading Serial Crystallography Collection<\/span><\/a><\/p>\n","protected":false},"author":2,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":{"footnotes":""},"class_list":["post-614","page","type-page","status-publish","hentry","entry"],"_links":{"self":[{"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/pages\/614","targetHints":{"allow":["GET"]}}],"collection":[{"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/users\/2"}],"replies":[{"embeddable":true,"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=614"}],"version-history":[{"count":7,"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/pages\/614\/revisions"}],"predecessor-version":[{"id":630,"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=\/wp\/v2\/pages\/614\/revisions\/630"}],"wp:attachment":[{"href":"https:\/\/bcsb.als.lbl.gov\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=614"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}